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primary antibody rat anti–mouse mac2  (Thermo Fisher)


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    Thermo Fisher primary antibody rat anti–mouse mac2
    Primary Antibody Rat Anti–Mouse Mac2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibody rat anti–mouse mac2/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    primary antibody rat anti–mouse mac2 - by Bioz Stars, 2026-03
    90/100 stars

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    ACKR2 controls the accumulation of macrophages in the mammary gland. (A) Flow cytometry was used to identify the percentage of CD11b + F4/80 + macrophages within the CD45 + population of the mammary gland during development. Gating based on fluorescence minus one (FMO) controls is shown. (B) FACS analysis of wild-type and Ackr2 −/− -deficient glands was carried out at 6, 6.5, 8 and 12 weeks. Results were combined from two independent experiments, n =8-11. (C) <t>Mac2+</t> cells, indicated by arrowheads, were visualised within the developing mammary gland by immunohistochemistry. Representative 40× bright-field images of Mac2-stained sections from wild-type and Ackr2 −/− glands are shown. (D,E) The number of Mac2+ cells per gland was the average counted from five individual FOV (40×) either (D) around TEBs or (E) from within adipose tissue, from 6 week ( n =3), 6.5 week ( n =5) and 8 week (wild type n =5, KO n =4) glands. Significantly different results are indicated. Error bars represent s.e.m.
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    primary antibody rat anti–mouse mac2  (Thermo Fisher)
    90
    Thermo Fisher primary antibody rat anti–mouse mac2
    ACKR2 controls the accumulation of macrophages in the mammary gland. (A) Flow cytometry was used to identify the percentage of CD11b + F4/80 + macrophages within the CD45 + population of the mammary gland during development. Gating based on fluorescence minus one (FMO) controls is shown. (B) FACS analysis of wild-type and Ackr2 −/− -deficient glands was carried out at 6, 6.5, 8 and 12 weeks. Results were combined from two independent experiments, n =8-11. (C) <t>Mac2+</t> cells, indicated by arrowheads, were visualised within the developing mammary gland by immunohistochemistry. Representative 40× bright-field images of Mac2-stained sections from wild-type and Ackr2 −/− glands are shown. (D,E) The number of Mac2+ cells per gland was the average counted from five individual FOV (40×) either (D) around TEBs or (E) from within adipose tissue, from 6 week ( n =3), 6.5 week ( n =5) and 8 week (wild type n =5, KO n =4) glands. Significantly different results are indicated. Error bars represent s.e.m.
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    https://www.bioz.com/result/primary antibody rat anti–mouse mac2/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    primary antibody rat anti–mouse mac2 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ACKR2 controls the accumulation of macrophages in the mammary gland. (A) Flow cytometry was used to identify the percentage of CD11b + F4/80 + macrophages within the CD45 + population of the mammary gland during development. Gating based on fluorescence minus one (FMO) controls is shown. (B) FACS analysis of wild-type and Ackr2 −/− -deficient glands was carried out at 6, 6.5, 8 and 12 weeks. Results were combined from two independent experiments, n =8-11. (C) Mac2+ cells, indicated by arrowheads, were visualised within the developing mammary gland by immunohistochemistry. Representative 40× bright-field images of Mac2-stained sections from wild-type and Ackr2 −/− glands are shown. (D,E) The number of Mac2+ cells per gland was the average counted from five individual FOV (40×) either (D) around TEBs or (E) from within adipose tissue, from 6 week ( n =3), 6.5 week ( n =5) and 8 week (wild type n =5, KO n =4) glands. Significantly different results are indicated. Error bars represent s.e.m.

    Journal: Development (Cambridge, England)

    Article Title: Atypical chemokine receptor ACKR2 controls branching morphogenesis in the developing mammary gland

    doi: 10.1242/dev.139733

    Figure Lengend Snippet: ACKR2 controls the accumulation of macrophages in the mammary gland. (A) Flow cytometry was used to identify the percentage of CD11b + F4/80 + macrophages within the CD45 + population of the mammary gland during development. Gating based on fluorescence minus one (FMO) controls is shown. (B) FACS analysis of wild-type and Ackr2 −/− -deficient glands was carried out at 6, 6.5, 8 and 12 weeks. Results were combined from two independent experiments, n =8-11. (C) Mac2+ cells, indicated by arrowheads, were visualised within the developing mammary gland by immunohistochemistry. Representative 40× bright-field images of Mac2-stained sections from wild-type and Ackr2 −/− glands are shown. (D,E) The number of Mac2+ cells per gland was the average counted from five individual FOV (40×) either (D) around TEBs or (E) from within adipose tissue, from 6 week ( n =3), 6.5 week ( n =5) and 8 week (wild type n =5, KO n =4) glands. Significantly different results are indicated. Error bars represent s.e.m.

    Article Snippet: Slides were blocked using a 20% goat serum (Vector Labs) before staining overnight at 4°C with a 1 in 6000 dilution in PBS containing 1% BSA (Sigma) of either primary rat anti-mouse Mac2 antibody (Cedarlane) or the isotype control antibody IgG2a (BD Biosciences).

    Techniques: Flow Cytometry, Fluorescence, Immunohistochemistry, Staining